Home | Contact
     ISSN 2149-2042
     e-ISSN 2149-4606
 
 
 
Volume : 35 Issue : 1 Year : 2020



Current Issue Archive Popular Articles Ahead of Print




Index












Membership




Applications



 
Aberrant SOCS3 Promoter Methylation as a Noninvasive Diagnostic Biomarker for Locally Advanced Prostate Cancer [Medeniyet Med J]
Medeniyet Med J. 2020; 35(2): 99-105 | DOI: 10.5222/MMJ.2020.58708  

Aberrant SOCS3 Promoter Methylation as a Noninvasive Diagnostic Biomarker for Locally Advanced Prostate Cancer

Berna Tan1, Turgay Turan2, Burcu Yucel1, Sedef Altundağ Kara4, Seda Salman Yılmaz3, Asif Yildirim2
1Istanbul Medeniyet University, Medical School, Department Of Medical Biology, Istanbul
2Istanbul Medeniyet University, Medical School, Department Of Urology, Istanbul
3Istanbul University, Cerrahpasa Medical School, Department Of Medical Genetics, Istanbul
4Istanbul Okan University, Medical School Department of Histology, Istanbul

Objective: The aim of this study was to investigate the promoter methylation status of Ras-associated domain family 1A (RASSF1A), O-6-methylguanine-DNA methyltransferase (MGMT), Phosphatase with tensin homology (PTEN) and Suppressor of cytokine signaling 3 (SOCS3) tumor suppressor genes and evaluate the clinical utility of these genes as non-invasive, blood-based epigenetic biomarkers for Prostate Cancer (PCa) diagnosis.
Methods: A total of 41 consecutive patients and 10 healthy control groups were enrolled. Pyrosequencing was performed to analyze the methylation levels of the promoter regions of the four tumor suppressor genes in patients compared to healthy controls.
Results: The promoter methylation levels of RASSF1A, MGMT, PTEN and SOCS3 did not differ between the patient and control groups. However, SOCS3 promoter methylation level was significantly higher for patients having locally advanced PCa compared to those having localized PCa (p<0.05).
Conclusion: Our results indicated that SOCS3 could be a useful, noninvasive blood-based epigenetic biomarker for the diagnosis of locally advanced PCa.

Keywords: Prostate cancer, DNA methylation, epigenetics, SOCS3


Lokal İlerlemiş Prostat Kanseri için Noninvaziv Diyagnostik Bir Biyobelirteç Olarak Sapmış SOCS3 Promotör Metilasyonu

Berna Tan1, Turgay Turan2, Burcu Yucel1, Sedef Altundağ Kara4, Seda Salman Yılmaz3, Asif Yildirim2
1İstanbul Medeniyet Üniversitesi Tıp Fakültesi Tıbbi Biyoloji Ana Bilim Dalı, İstanbul
2İstanbul Medeniyet Üniversitesi Tıp Fakültesi Üroloji Ana Bilim Dalı, İstanbul
3İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi Tıbbi Genetik Ana Bilim Dalı, İstanbul
4Istanbul Okan Üniversitesi, Tıp Fakültesi, Histoloji Anabilim Dalı, İstanbul

Amaç: Bu çalışmanın amacı tümör baskılayıcı genler Ras-associated domain family 1A (RASSF1A), O-6-methylguanine-DNA methyltransferase (MGMT), Phosphatase with tensin homology (PTEN) ve Suppressor of cytokine signaling 3 (SOCS3)’in promotör metilasyonlarını araştırmak ve bu genlerin Prostat Kanseri (PK) tanısı için noninvazif, kan temelli epigenetik biobelirteçler olarak klinik yararını değerlendirmekti.
Metod: 41 hasta ve 10 sağlıklı kontrol grubu çalışmaya kaydedildi. Dört tümör baskılayıcı genin promotör bölgelerinin metilasyon düzeylerini hastalarda sağlıklı kontrol grubuna kıyasla analiz etmek için Pyrodizileme gerçekleştirildi.
Sonuçlar: RASSF1A, MGMT, PTEN ve SOCS3 promotör metilasyon düzeyleri hasta ve konrol grubu arasında farklı değildi. Ancak SOCS3 promotör metilasyon düzeyi lokalize PK’lı hastalara kıyasla lokal olarak ilerlemiş PK’lı hastalarda önemli şekilde daha yüksekti (p<0.05).
Sonuç: Sonuçlarımız SOCS3’ün lokal olarak ilerlemiş PK tanısında elverişli, noninvazif kan temelli epigenetik biobelirteç olabileceğini gösterdi.

Anahtar Kelimeler: Prostat kanseri, DNA metilasyonu, epigenetikler, SOCS3


Berna Tan, Turgay Turan, Burcu Yucel, Sedef Altundağ Kara, Seda Salman Yılmaz, Asif Yildirim. Aberrant SOCS3 Promoter Methylation as a Noninvasive Diagnostic Biomarker for Locally Advanced Prostate Cancer. Medeniyet Med J. 2020; 35(2): 99-105

Corresponding Author: Berna Tan, Türkiye


TOOLS
Full Text PDF
Print
Download citation
RIS
EndNote
BibTex
Medlars
Procite
Reference Manager
Share with email
Share
Send email to author

Similar articles
PubMed
Google Scholar





 

  Copyright © 2019 MEDJ All Rights Reserved