Antioxidant activity and cytotoxic effects of Prunus spinosa L. fruit extract on various cancer cell lines

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Antioxidant activity and cytotoxic effects of Prunus spinosa L. fruit extract on various cancer cell lines [Medeniyet Med J]

Medeniyet Med J. 2019; 34(3): 297-304 | DOI: 10.5222/MMJ.2019.87864

Antioxidant activity and cytotoxic effects of Prunus spinosa L. fruit extract on various cancer cell lines

Nihal Karakas¹, Mehmet Evren Okur², Irem OZTURK³, Sule Ayla⁴, Ay�e Esra Karada�⁵, Derya �i�ek Polat⁶
¹Istanbul Medipol University, School of Medicine, Department of Medical Biology, Beykoz/�stanbul, Turkey
²University of Health Sciences, Faculty of Pharmacy, Department of Pharmacology, �sk�dar/�stanbul, Turkey
³Istanbul Medipol University, Regenerative and Restorative Medicine Research Center, Beykoz/�stanbul, Turkey
⁴Istanbul Medipol University, School of Medicine, Department of Histology And Embryology, Beykoz/�stanbul, Turkey
⁵Istanbul Medipol University, School of Pharmacy, Department of Pharmacognosy, Beykoz/�stanbul, Turkey
⁶Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Botany, Tando�an/Ankara, Turkey

Aim Prunus spinosa L. (Rosaceae) fruits are consumed as food in Turkey. This study was aimed to evaluate P. spinosa methanol extract antioxidant activity and cytotoxic effects on cancer cell lines.
Methods P. spinosa fruit methanol extract was evaluated for its in vitro cytotoxic activity by using glioblastoma multiform (GBM) brain cancer (LN229, U87 and T98G) and pancreas cancer (PANC-1 and AsPC-1) cell lines. Cell viability assays were performed through measuring the percentage of viable cells using a luminescence system, and the antioxidant activities by ABTS and DPPH radical scavenging spectrophotometrically. Differences were considered as statistically significant at p*<0.001 and p**<0.0005 according to unpaired student t-test.
Results P. spinosa fruit methanol extract showed 2548�17,74 mg GAE/100 g corresponding to the total phenolic content, and moderate antioxidant activity (0,1896 � 0,1143 and 0,0729 � 0,0348) by ABTS� and DPPH� assays. To the best of knowledge, after of evaluation the results of brain and pancreas cancer cell lines, significant cytotoxic activities with 50-63% cell viability of GBM brain cancer cells were determined while no cytotoxicity was observed on pancreas cancer cell lines, PANC-1; and AsPC-1.
Conclusion The results of this study showed that the P. spinosa fruit methanol extract has significant antioxidant capacity and leads to statistically significant decreased viability on glioblastoma brain cancer cells.

Keywords: Prunus spinosa, Blackthorn, cytotoxicity, cancer, antioxidant activity

Prunus spinosa L. Ekstresinin �e�itli Kanser H�cre Soylar�ndaki Antioksidan ve Sitotoksik Etkileri

Nihal Karakas¹, Mehmet Evren Okur², Irem OZTURK³, Sule Ayla⁴, Ay�e Esra Karada�⁵, Derya �i�ek Polat⁶
¹�stanbul Medipol �niversitesi, T�p Fak�ltesi, T�bbi Biyoloji Anabilim Dal�, Beykoz/�stanbul, T�rkiye
²Sa�l�k Bilimleri �niversitesi, Eczac�l�k Fak�ltesi, Farmakoloji Anabilim Dal�, �sk�dar/i�stanbul, T�rkiye
³�stanbul Medipol �niversitesi, Rejeneratif ve Restoratif T�p Ara�t�rmalar� Merkezi, Beykoz/�stanbul, T�rkiye
⁴�stanbul Medipol �niversitesi, T�p Fak�ltesi, Histoloji Ve Embriyoloji Anabilim Dal�, Beykoz/�stanbul, T�rkiye
⁵�stanbul Medipol �niversitesi, Eczac�l�k Fak�ltesi, Farmakognozi Anabilim Dal�, Beykoz/�stanbul, T�rkiye
⁶Ankara �niversitesi, Eczac�l�k Fak�ltesi, Farmas�tik Botanik Anabilim Dal�, Tando�an/Ankara, T�rkiye

Ama� �akal eri�i (Prunus spinosa), g�lgiller (Rosaceae) familyas�ndan bir a�a�c�k t�r�d�r ve T�rkiye�de meyvesi besin olarak t�ketilmektedir. Bu �al��ma P. spinosa metanol ekstresinin antioksidan aktivitesini ve kanser h�cre hatlar� �zerindeki sitotoksik etkilerini de�erlendirmeyi ama�lam��t�r.
Y�ntem P. spinosa meyvesi metanol ekstresi, glioblastoma multiform (GBM) beyin kanseri (LN-229, U-87 ve T98G) ve pankreas kanseri (PANC-1 ve AsPC-1) h�cre hatlar� kullan�larak in vitro sitotoksik aktivitesi ara�t�r�lm��t�r. H�cre canl�l�� deneyleri, biyol�minesans sistemi kullan�larak canl� h�crelerin y�zdesinin ve antioksidan aktivitelerinin, spektrofotometrik olarak ABTS ve DPPH radikalleri ile �l��lmesi yoluyla ger�ekle�tirilmi�tir. �statiksel anlaml�l�k, unpaired student t-test ile belirlenmi�tir (p*<0.001 and p**<0.0005)
Bulgular P. spinosa meyve metanol ekstresi, ABTS� ve DPPH� testlerinde toplam fenolik i�eri�e kar��l�k gelen 2548 � 17,74 mg GAE / 100 g ve orta d�zeyde antioksidan aktivite (0,1896 � 0,1143 ve 0,0729 � 0,0348) g�stermi�tir.
Sonu� Elde etti�imiz bilgiler ��nda, beyin ve pankreas kanseri h�cre hatlar�ndaki sonu�lar�n�n de�erlendirilmesinden sonra, GBM beyin kanseri h�crelerinde % 50-63 aras�ndaki h�cre canl�l�� ile �nemli derecede sitotoksik aktivitesi belirlenmi� ancak PANC-1 ve AsPC-1 pankreas kanseri h�cre hatlar�nda sitotoksisite g�zlenmemi�tir,. Sonu� olarak, P. spinosa meyvesi metanol ekstresinin �nemli antioksidan kapasiteye sahip oldu�u ve glioblastoma beyin kanseri h�crelerinin can�l�l��nda istatistiksel olarak anlaml� bir azalmaya yol a�t�� g�sterilmi�tir.

Anahtar Kelimeler: Prunus Spinosa, �akal eri�i, sitotoksisite, kanser, antioksidan aktivite

Nihal Karakas, Mehmet Evren Okur, Irem OZTURK, Sule Ayla, Ay�e Esra Karada�, Derya �i�ek Polat. Antioxidant activity and cytotoxic effects of Prunus spinosa L. fruit extract on various cancer cell lines. Medeniyet Med J. 2019; 34(3): 297-304

Corresponding Author: Nihal Karakas, T�rkiye

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