Smoking Induces the Circulating Levels of Matrix Metalloproteinase -9 and its Association with Cardiovascular Risk in Young SmokersDinesh NATH, Meera SHIVASEKAR, V. M. VINODHINI
SRM Medical College Hospital and Research Centre, SRMIST, Department of Biochemistry, Kattankulthur, Tamil Nadu, India
Objective: Smoking causes cardiovascular risk which may alter the stability between the production and degradation of the extracellular matrix. Matrix metalloproteinase-9 (MMP-9) is a zinc-containing endopeptidase that degrades the extracellular matrix and plays a vital role in tissue remodeling and contributes to a variety of physiological processes. As a result, elevated serum MMP-9 levels produced by smoking, particularly at a younger age, raise the risk of future CHD. So the study aims to find out the possible relationship between smoking-induced circulating MMP-9 and the risk of cardiovascular disease in young smokers. Keywords: APO-E, Cardiovascular disease, hsCRP, Matrix metalloproteinase-9, Smoking
Methods: The cross-sectional study consisted of three groups. Group 1 consisted of 120 healthy non-smokers; Group 2 consisted of 120 smokers with coronary heart disease (CHD), whereas Group 3 consisted of 120 smokers with CHD and diabetes who came to the SRM Medical College Hospital in Tamil Nadu for cardiology and medical OP in the age group between 20-55 years. Serum MMP-9, hs-CRP, and APO-E levels were measured using enzyme-linked immunosorbent assay (ELISA) method and the lipid levels were measured enzymatically using AU480 automatic analyzer (backman coulter). Results: The mean serum MMP-9, hs-CRP, and APO-E levels were significantly higher in both groups (p<0.001) when compared to controls. The study also shows a significant positive association between MMP-9 with hs-CRP, APO-E, smoking burden, and smoking intensity. Conclusion: The study concludes a significant association exists between cigarette smoking with MMP-9 and also relative exposure to circulating inflammation markers plays a potential role in the pathogenesis of CHD.
Corresponding Author: Meera SHIVASEKAR, India